plasmid-based

plasmid-based
мед.нареч. на основе плазмидного вектора

Англо-русский медицинский словарь. 2012.

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  • Plasmid — Figure 1: Illustration of a bacterium with plasmid enclosed showing chromosomal DNA and plasmids. In microbiology and genetics, a plasmid is a DNA molecule that is separate from, and can replicate independently of, the chromosomal DNA …   Wikipedia

  • Plasmid preparation — Plasmid miniprep. 0.8% agarose gel ethidium bromide stained. A plasmid preparation is a method used to extract and purify plasmid DNA. Many methods have been developed to purify plasmid DNA from bacteria. These methods invariably involve three… …   Wikipedia

  • Plasmid stabilisation technology — Introduction In Molecular Biology and Biotechnology, Plasmid stabilisation technologies are an important issue in particular for the production of recombinant proteins in procaryotic hosts. Technologies using recombinant DNA have become of… …   Wikipedia

  • plasmid prep — Generic term for the isolation of recombinant plasmids from liquid bacterial culture, usually by alkaline/detergent lysis, selective precipitation of other components, and affinity purification of plasmid. As this is the most exciting thing most… …   Dictionary of molecular biology

  • Column-based nucleic acid purification — is a solid phase extraction method to quickly purify nucleic acids. This method relies on the fact that the nucleic acid may bind (adsorption) to the solid phase (silica or other) depending on the pH and the salt content of the buffer, which may… …   Wikipedia

  • Spin column-based nucleic acid purification — Silica on a minicolumn with water and with DNA sample in chaotropic buffer Column based nucleic acid purification is a solid phase extraction method to quickly purify nucleic acids. This method relies on the fact that the nucleic acid may bind… …   Wikipedia

  • conjugative functions — Plasmid based genes and their products that facilitate the transfer of a plasmid from one bacterium to another via conjugation …   Glossary of Biotechnology

  • Zinc finger nuclease — Zinc finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA binding domain to a DNA cleavage domain. Zinc finger domains can be engineered to target desired DNA sequences and this enables zinc finger… …   Wikipedia

  • Ventilator-associated pneumonia — (VAP) is a sub type of hospital acquired pneumonia (HAP) which occurs in people who are on mechanical ventilation through an endotracheal or tracheostomy tube for at least 48 hours. VAP is a medical condition that results from infection which… …   Wikipedia

  • Telomerase — is an enzyme that adds specific DNA sequence repeats ( TTAGGG in all vertebrates) to the 3 ( three prime ) end of DNA strands in the telomere regions, which are found at the ends of eukaryotic chromosomes. The telomeres contain condensed DNA… …   Wikipedia

  • DNA vaccination — The making of a DNA vaccine. DNA vaccination is a technique for protecting an organism against disease by injecting it with genetically engineered DNA to produce an immunological response. Nucleic acid vaccines are still experimental, and have… …   Wikipedia


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